| 摘要 |
PURPOSE:To obtain an antitumor active polypeptide having improved activity and stability, widened reaction spectrum and reduced side effects, having a specific amino acid sequence. CONSTITUTION:For example, a base sequence of a structural gene of human TNF precursor cDNA of Pennica as a foundation is provided with a breakage by a proper restriction enzyme at a fitting position, with an initiation code ATG at the 5' side and and two translation completion codons TG and TAA at the 3' side, respectively. The upper stream of the initiation codon is provided with a breakage site by restriction enzyme ClaI and can be bonded to a promoter in a shape wherein space between SD sequence and the initiation codon is maintained in a proper state. The downstream of the two translation completion codons is supplied with a breakage site by restriction enzyme HindIII and the sequence can be readily linked to a vector plasmid. Consequently, the aimed antitumor active polypeptide having an amino acid sequence shown by the formula is obtained. |
