Method for the immunoassay of apolipoproteins.

摘要

Using serial dilutions of Apo AI - Apo B standard serum the maximum sensitivity value found for Apo AI determination is 1 mu g/ml and the minimal sample volume necessary to perform one determination in double is 2 mu l. For Apo B determination the maximum sensitivity value is 1.2 mu g/ml and, also in this case, the minimal sample volume for one determination in double is 2 mu l. The same procedure can be used for the determination of other apolipoproteins (AII, AIV, CI, CII, CIII, D, E). A computation of their plasma levels and ratios is a more valuable indicator of cardiovascular risk than the current hyperlipidemic indexes. The method according to the present invention can be named: RIECA ( Recombinant Immuno-Enzymatic Competition Assay).