Cloning method and expression of genes constituting functional organised structures.

摘要

The transformation of cells of a receptor microorganism strain by introducing DNA fragments consisting of, or comprising, at least two interchangeable foreign genes and capable of expressing in the receptor cells proteins constituting organised functional structures. The indicator gene is capable of expressing a protein which makes it possible to detect the biological activity of the protein expressed by the gene to be disclosed, this activity resulting from the interaction between the proteins. The biological activity of the required protein is detected by the use of a physicochemical means. This process is advantageously applied to the production, for example, of adenylate cyclase or of calmodulin.