摘要 |
Hybridoma cell line consists of (1), as immortalised cell component, a xenogenetic Hybridoma which has lost (or lacks) ability to produce a particular material, fused to (2) a cell which is able to produce a specific cpd. (A). Component (2) is genetically compatible with the non-transformed partner in the xenogenetic hibridoma. Component (2) and the non-transformed partner in (1) are from the same species, esp. human. (2) is particularly a lymphocyte which has been pre-sensitised to produce (A). USE/ADVANTAGE - When (1) is used as immortalising component, the final hybridomas have higher stability, provide higher (A) yields; grow rapidly and are easy to clone.
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