| 摘要 |
A method is disclosed for isolating a mutant microorganism. The method includes the following steps: (1) separately microencapsulating individual or small numbers of a microorganism population containing the mutant to obtain a first microdroplet; (2) thereafter surrounding the first microdroplet, with or without an outer semi-permeable membrane, with an outer gel-coating to obtain a gel-coated microdroplet, wherein the gel layer contains an indicator material which reacts to the presence of the mutant microorganism; (3) culturing the resultant microcapsules so that the microdroplets containing mutant microorganism are able to induce a detectable difference in the indicator material from microdroplets, containing non-mutant microorganisms; and (4) separating the microdroplets containing mutant microorganisms from those containing non-mutant microorganisms based upon the differences in the indicator material.
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