| 摘要 |
PURPOSE:To produce a heat-resistant neutral protease in high efficiency, by linking a heat-resistant neutral protease gene DNA with a replicable vector plasmid, preparing a transformant with the obtained recombinant plasmid and culturing the transformant in a nutrient medium. CONSTITUTION:A vector plasmid replicable in a host cell is integrated with a heat-resistant neutral protease gene DNA prepared from a microorganism capable of producing a heat-resistant neutral protease. The obtained recombinant plasmid is transformed into a host cell to obtain a transformant (pSP53). The transformant is cultured in a medium produced by compounding a carbon source, a nitrogen source, other nutrients, stabilizer for plasmid, etc., to produce and accumulate a heat-resistant neutral protease in the cultured product. The preparation process of pSP53 and a restriction enzyme map are shown in the figure. |
