发明名称 |
STABLE, SINGLE-LIQUID ALPHA-AMYLASE REAGENT |
摘要 |
A stable, single liquid alpha-amylase assay reagent comprises a polysaccharide or long chain oligosaccharide substrate for alpha-amylase having an optically detectable label bonded to the reducing end glucose, by a bond cleavable by alpha- or beta-glucosidase, and a blocking group bonded to the terminal end glucose. The reagent further comprises beta-amylase and either alpha- or beta-glucosidase. Sorbitol is provided in an amount of about 5 % to retard degradation of the enzymes. Zwitterionic buffers are also provided to stabilize the enzymes. In preparing the reagent, the enzymes and substrate are filtered through a 0.2 micron or less filter to remove alpha-amylase producing organisms. |
申请公布号 |
WO8900600(A1) |
申请公布日期 |
1989.01.26 |
申请号 |
WO1988US02414 |
申请日期 |
1988.07.15 |
申请人 |
MODROVICH, IVAN, ENDRE |
发明人 |
MODROVICH, IVAN, ENDRE;KWAN, SHING, SAI;WORTHAM, LEON, CHARLES |
分类号 |
C12Q1/34;C12Q1/40;(IPC1-7):C12N9/96;C12Q1/54 |
主分类号 |
C12Q1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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