| 摘要 |
<p>A method for increasing the translation efficiency of a mRNA sequence is provided. In certain of its preferred embodiments, the method comprises the steps of: (a) constructing a predicted secondary structure for the mRNA; (b) analyzing the predicted secondary structure to determine if either or both of the AUG initiation codon and the Shine-Dalgarno sequence is contained in a double stranded portion of a stem-loop region of the predicted secondary structure; (c) calculating a free energy value for the stem-loop region; and (d) if the calculated free energy value is in the range of from zero to about -10.0 kcal/mole, modifying the DNA sequence for the mRNA so that when the modified sequence is transcribed it produces a modified mRNA sequence which has a predicted secondary structure wherein the AUG initiation codon and the Shine-Dalgarno sequence are not included in a double stranded portion of a stem-loop region of the predicted secondary structure. By means of this method, ten-fold increases in protein production have been achieved.</p> |
