TIME-RESOLVED FLUORESCENCE APPARATUS AND IMMUNOASSAY

摘要

A method and apparatus for time-resolved fluorescence spectroscopy is described in which laser light from a single pulse is used to excite fluorescent photons in a sample (24), which fluorescence is detected by a PMT (14) optimized for linearity and response time to produce photoelectrons which generate a current at the PMT anode. This current is discharged through an R/C network to produce a voltage amplitude waveform which is converted to an optical image (26), intensified (28), stored (36) and digitized (34). The digitized version of the optical image is processed in a data processor (38) to calculate the true fluorescence impulse response. Fluorescence immunoassay for determining single or multiple analytes based upon a single measurement of fluorescence are described.