Human pancreatic secretory trypsin inhibitor.

摘要

A human pancreatic secretory trypsin inhibitor (PSTI) essentially free of other proteins of human origin is produced by using recombinant DNA techniques. The human PSTI is prepared by transforming E. coli into a transformant capable of producing the human PSTI, cultivating the transformant to produce the human PSTI, and recovering the human PSTI from the culture supernatant. The transformant may preferably be prepared by transforming a cell wall lipoprotein-deleted mutant E. coli strain by using a vector replicable in E. coli. The vector may preferably comprise a DNA sequence coding for the human PSTI, as well as a promotor, an SD sequence, and a DNA sequence coding for a signal peptide. The present invention also relates to a process for producing the human PSTI, a vector useful in the process, and an E. coli transformant having the vector.