| 摘要 |
PURPOSE: To obtain a reagent system used for measuring the hemoglobin content of whole blood by using an aqueous polyvinyl pyrrolidone (PVP) solution having a pH value sufficient to modify hemoglobin as a reagent. CONSTITUTION: A PVP polymer having a molecular weight of about 10,000 to 360,000 is dissolved in an aqueous solution having a pH value of >=8, preferably, a value within a desirable range from about 12 to 14 which is sufficient to modify hemoglobin contained in a blood sample. In a method for measuring hemoglobin content of whole blood, the hemoglobin content is measured by reading the absorbancy of a blood sample at a wavelength of about 575nm mixed with the basic aqueous PVP solution. About 5- to 10-ml basic aqueous solution is mixed with 100-μl whole blood. After the blood sample is treated with a reagent system, the absorbancy of the reaction product at a wavelength of about 575nm is read by using water as a blank. The reading of the absorbancy of the sample is in direct proportional to the hemoglobin content of the sample.
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