POLYPEPTIDE HAVING ANTI-BLOOD COAGULATING ACTION

摘要

PURPOSE:To safely and stably feed an anti-blood coagulating substance (PCI) polypeptide derived from a human placenta cDNA library at a low price and large quantities, by using a genetic engineering method using a recombinant vector recombined by DNA fragment coding PCI polypeptide. CONSTITUTION:A transformer cell retaining a recombinant vector consisting of DNA fragment having base sequence capable of coding a specific amino acid sequence thereto and/or DNA fragment having complementary base sequence and a vector capable of replicating is cultivated and the aimed polypeptide produced from the cultivated product is collected. In the above-mentioned method, the DNA fragment coding PCI polypeptide is obtained from a human placenta dDNA library by utilizing a PCI specific antibody as a probe.