PRODUCTION OF PHYSIOLOGICALLY ACTIVE PEPTIDE CONTAINING CYSTEINE RESIDUE

摘要

PURPOSE:To efficiently mass produce a desired physiologically active peptide containing cysteine residue, by selecting an optimum addition peptide. CONSTITUTION:Escherichia coli transformed with a plasmid capable of manifesting a fused protein shown by the formula [B is desired physiologically active peptide containing cysteine residue; A is polypeptide (addition polypeptide) which comprises 90-220 amino acid residues and contains no cysteine residue; L is amino acid residue (linker) to bond C-end of the component A to N-end of the component B] under control of a promoter derived from Escherichia coli or a promoter derived from phage gene is cultivated. The culture cell is ground and an insoluble fraction of the ground cell is treated with a solubilizer to give a solubilized fused protein. The fused protein is treated with an enzyme or a chemical substance, desired physiologically active peptide B is separated from the fused protein and collected.