| 摘要 |
<p>Use of extract from bacteria for determination of HDL2 and HDL3 concentration in serum, plasma or other solutions containing HDL. The extract is produced from bacteria grown on solid nutrient medium containing more than 10 mg/l of cystein, or on solid nutrient medium in an atmosphere with increased content of CO2. Extract may also be produced by growing the bacteria in liquid nutrient medium containing cystein. After growth, bacteria are removed, and the medium is concentrated, dialyzed and used as extract. The extract is added to melted agar that is poured into Petri dish (1). After solidification of agar with extract, wells (3) are made in the agar. The wells are filled with the HDL samples. After a diffusion time of 15-20 hours, a precipitation ring will be formed around the wells (4). Diameter of the ring is a function of HDL2 concentration in the sample. HDL3 concentration is calculated as the difference between total HDL (determined with known methods) and HDL2. Extract may also be added to dilutions of serum and the precipitation reaction in the solution may be read by use of nephelometry or turbidimetry.</p> |
