Immunoassay method and kit for detecting hapten by agglutination.

摘要

A method for detecting a hapten in a serum, plasma or blood sample which comprises the steps of: a) admixing the sample, in any order, with: 1) a first reagent comprising particles bearing the hapten, or an analog thereof, suspended in an aqueous medium, and 2) a second reagent comprising a monoclonal antibody of the IgA class against the hapten in such an amount and of such an activity sufficient to agglutinate the particles in the absence of the hapten in the sample, but to cause reduced amounts of agglutination with increasing amounts of hapten in the sample; and b) detecting the degree of agglutination as a measure of the presence and amount of hapten in the sample.