IN VITRO CELL CULTURE SYSTEM

摘要

In vitro cell culture systems for anchorage-dependent mammalian cells (B) using solid substrates (A) of mitogenic calcium compounds, such as hydroxyapatite and tricalcium phosphate forms of calcium phosphate and calcium carbonate. The calcium solid substrates (A) can be in the form of granules with a particle size of at least 0.050 mm. or in the form of solid bodies and may be either porous or non-porous. Unique features of cells (B) cultured in the stated in vitro cell culture systems include the growth of cells in layers many cells thick, growth of cells that maintain their phenotype and the ability to culture cells for extended periods of time.