| 摘要 |
PURPOSE:To concentrate an unsaturated fatty acid having an unsaturated bond at a specific position in a state of glyceride, by selecting a lipase having low hydrolyzing activity to unsaturated fatty acids and hydrolyzing an oil or fat using the lipase. CONSTITUTION:An oil or fat containing unsaturated fatty acid having unsaturat ed bond at or after DELTA6 position, e.g. seed oil of evening pimrose is hydrolyzed with a lipase having the following property. When an oil or fat A containing petroselinic acid as a main constituent fatty acid and an oil or fat B free from a fatty acid having an unsaturated bond at or after DELTA6 position as main constitu ent fatty acid are hydrolyzed with the lipase, the difference between the hydroly sis ratio of A and B is >=10%. The hydrolysis is carried out at 30-37 deg.C for 0.5-5hr using 50-500 IU of lipase per 1g of the oil or fat and 1-50pts. of water per 1pt. of the oil or fat. The unsaturated fatty acid having unsaturated bond at or after the DELTA6 position is concentrated in the state of glyceride.
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