| 摘要 |
PURPOSE:To increase permissibility to insertion of exogenous DNA and improve efficiency of transformation, by forming a self-duplicable plasmid vector in the tissues in Escherichia coli and Bacillus subtilis. CONSTITUTION:The 105 kb DraI-EcoRI fragment containing the region controlling self-duplication function of pUC19 and the multiple cloning site is connected to the 3.6 kb EcoRI-Pvu II fragment containing the region controlling self- duplication function of pUB110 and the kanamycin nucleotidyl transferase gene. Thus, 4.7 kb plasmid vector can be formed. The plasmid vector has many recognition cleavage sites for restriction enzymes, to increase permissibility for outer DNA insertion, which is maintained stably in the host cells, resulting in high transformation efficiency. |
