| 摘要 |
PURPOSE:To prepare L-phenylalanine, economically, by culturing a specific mutant belonging to Bacillus genus and capable of producing L-phenylalanine aerobically in a liquid medium, and collecting the produced and accumulated L- phenylalanine. CONSTITUTION:A mutant (e.g. Bacillus subtilis FERM-P 6759 and Bacillus subtilis FERM-P 6760) resistant to phenylalanine analogue, demanding L-tyrosine for growth, producing L-phenylalanine, and preferably resistant to sulfa drugs, is prepared by the mutagenic treatment of wild strain of Bacillus subtilis. The mutant is cultured aerobically in a liquid nutrient medium containing L-tyrosine, and after the cultivation, the produced and accumulated phenylalanine is separated from the culture liquid, e.g. by ion exchange resin treatment. |
