METHODS FOR PROTEIN BINDING ENZYME COMPLEMENTATION ASSAYS.

摘要

Improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by recombinant DNA techniques, DNA synthesis or chemical polypeptide synthesis techniques which are capable of interacting to form an active enzyme complex having catalytic activity characteristics of beta -galactosidase is described. Both homogeneous and heterogeneous assays utilizing these polypeptides are described.