发明名称 |
PRODUCTION OF DOUBLE STRANDED DNA |
摘要 |
It is possible by synthesis of a DNA single strand with a hairpin structure at one or both ends, filling in to give the double strand and opening or elimination of the hairpin structure(s) to obtain even very long double-stranded DNA fragments or genes. It is also possible to attach mutagenic primers to the single strand and thus to carry out site-specific mutagenesis. Furthermore, the preparation of highly labelled DNA sequences is possible in the filling-in reaction. <IMAGE> |
申请公布号 |
JPS63304989(A) |
申请公布日期 |
1988.12.13 |
申请号 |
JP19880125616 |
申请日期 |
1988.05.23 |
申请人 |
HOECHST AG |
发明人 |
OIGEN UURUMAN;FURIIDORITSUHI HAIN |
分类号 |
C12N15/09;C07H21/04;C07K14/585;C12N1/20;C12N1/21;C12N15/10;C12P19/34;C12P21/02;C12Q1/68;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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