| 摘要 |
PURPOSE:To determine the quantity of glucose from the determined quantity of hydrogen peroxide produced by enzymatic decomposition of glucose in a specimen using a calibration curve, by treating a biospecimen with an anion exchange resin and then with a reducing agent prior to the enzymatic treatment and decomposing existing hydrogen peroxide in the presence of a catalyst. CONSTITUTION:A specimen such as urine, blood, serum, plasma, lymph, saliva and gastric juice is treated with a strongly basic anion exchange resin (OH<->-type resin) having particle size of 100-200 mesh and then with a reducing agent such as sodium borohydride or sodium sulfite to effect the reduction and decomposition of the hydrogen peroxide existing in the treated specimen. The glucose in the specimen is oxidized and decomposed with a glucose oxidase to produce hydrogen peroxide. The hydrogen peroxide is determined by the measurement of chemiluminescence emitted by the addition of luminol and potassium ferricyanide or chemical or physical methods and the glucose concentration in the specimen is determined from the amount of hydrogen peroxide based on a calibration curve.
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