Process for integration of a known DNA sequence in ascosporogenic yeasts, vectors used and new yeast strains

摘要

According to the invention, a new yeast strain is obtained by integration of an integrative vector containing a gene encoding a specific protein, especially the gene MEL1, at the locus of a cryptic gene, especially the gene for beta -glucosidase, of an ascosporogenic yeast. Application especially to the construction of bread-making yeast strains having a melibiase activity independent of the galactose system. The segment EcoRI EcoRI represented in Figure 9 is an example of an integrative vector which can be used.