CATALYST TRANSFORMATION BY PROTEIN PHOSPHORYLATION AS A MEANS FOR ENZYME IMMUNOASSAY

摘要

A test composition for use in a specific binding enzyme immunoassay for detecting an analyte in a sample, includes: (a) a target protein capable of mediating a signal generating process, the mediating activity of the target protein being changed by covalently bonding a regulatory group thereto; (b) a regulatory enzyme capable of catalyzing the covalent bonding of the regulatory group to the target protein; (c) a conjugate of a substrate molecule for the regulatory enzyme and an analyte, the substrate containing the regulatory group; (d) a specific binding partner for the analyte; and (e) reagent means for generating a detectable signal responsive to the condition of the target protein.