发明名称 |
Autocatalytic replication of recombinant RNA |
摘要 |
This invention concerns recombinant RNA molecules comprising a recognition sequence for the binding of an RNA-directed RNA polymerase, a sequence for the initiation of product strand synthesis and a heterologous sequence of interest inserted at a specific site in the internal region of the recombinant molecule. Such recombinant RNA molecules are capable of serving as a template for the synthesis of complementary single-stranded molecules by RNA-directed RNA polymerase. The product molecules so formed are also capable of serving as a template for the synthesis of additional copies of the original recombinant RNA molecule. In a preferred embodiment of the invention Q beta replicase is used as the RNA-directed RNA polymerase.
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申请公布号 |
US4786600(A) |
申请公布日期 |
1988.11.22 |
申请号 |
US19840614350 |
申请日期 |
1984.05.25 |
申请人 |
THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK |
发明人 |
KRAMER, FRED R.;MIELE, ELEANOR A.;MILLS, DONALD R. |
分类号 |
C12N15/10;C12P19/34;C12Q1/68;(IPC1-7):C12P19/34;C12P21/00;C12N15/00;C07H15/12 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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