| 摘要 |
PURPOSE:To enable to stably determine free fatty acid in a specimen, especially free fatty acid in blood on clinical inspection in good precision, by using a specific fatty acid or water soluble salt thereof as a part of reagents. CONSTITUTION:The first reagent containing at least ATP, CoA and acyl CoA synthesizing enzyme is reacted with a free fatty acid in a specimen to afford CoA. Then the second reagent containing a fatty acid expressed by formula I (R is 1-20C aliphatic group; n is integer of 0, or 1-2; A is -CH=CH- or -CHY-CHZ-; Y and Z are H, hydroxyl group or lower alkyl group) or water soluble salt thereof and acyl CoA oxidation enzyme and as necessary further reagent for measuring hydrogen peroxide by colorimetric method is reacted with the above-mentioned acyl CoA and change caused by oxidation of acyl CoA, e.g. produced hydrogen peroxide is measured.
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