| 摘要 |
PURPOSE:To improve adhesiveness to cells, extension of cells and to improve multiplication and activity maintenance, by coating a high polymer substrate with a film of a polymerized organosilicon compound by plasma treatment and using the coated high polymer substrate as a substrate for cell culture. CONSTITUTION:A pair of facing electrodes connected to a high-frequency electric source are set in a reactor consisting of a bell-jar and a high polymer substrate is supported between the electrodes. Then, the bell-jar is sufficiently evacuated and a gaseous organosilicon compound is introduced into the bell-jar. Plasma is generated between the electrodes to form a plasma polymerized film of the organosilicon compound on the surface of the high polymer substrate. A porous high polymer material is preferable as the high polymer substrate and tubular or hollow shape is preferable as the shape for high-density culture. The prepared substrate for cell culture is useful for production system of hormone, etc., by cultivation of animal cell.
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