| 摘要 |
PURPOSE:To accurately measure activity even with respect to a cell slow in motion and a change speed such as a fertilized egg, by converting the magnified image of a cell to an electrical image signal by an image pickup means. CONSTITUTION:At first, a laboratory dish 11 having a fertilized egg arranged thereto in the state immersed in a preserving liquid is arranged to the measuring stage of an inverted microscope 1 and the fertilized egg is held by a micro- manipulator 12 so as not to move to perform sample setting. Next, the focus of a CCD camera 2 is controlled to start the processing due to an image processing apparatus 3. At this time, the image of the fertilized egg is inputted as a master image at first and stored. Thereafter, the image of the fertilized egg after the elapse of a predetermined time is inputted to perform the image operation due to the digital subtraction processing of both images. The image obtained as a result becomes a deep-black image with respect to a part generating no change at all and the image of a part generating change becomes gradually bright according to the degree of the change. The number of pixels are counted at every gray level to form a histogram at every gray level and the degree of the activity of the fertilized egg is calculated.
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