| 摘要 |
PURPOSE:To enable massproduction with compactization, by forming two insulated electrodes on the top surface of a planar body from internal surfaces of recesses formed over one surface thereof to immobilize hydrophilic independent nurse bacteria therein. CONSTITUTION:After recesses 5 are formed on an Si substrate 2 on which an SiO2 film 3 is formed and the internal surface thereof is covered with an SiO2 film 6. Then, after the formation of positive and negative electrodes 7 and 8, independent nurse bacteria 10 are placed into the recesses 5 to make carbon dioxide conductive as suspended in a physiological salt water and a thermosetting resin is dropped to form an immobilizing film 16 for immobilization of microorganism. Then, an Si rubber or the like is applied to form a water repelling film 17. In the measuring of the concentration of carbon dioxide using an electrode thus obtained, the electrode is immersed into a buffer to stabilize and then, a sample liquid is added into the buffer to detect changes in a detection value. This compactizes the electrode in dimensions, thereby enabling massproduction with ease of use. |
