发明名称 |
Isolation of fibroblast growth factor |
摘要 |
Basic Fibroblast Growth Factor (FGF) is substantially purified by the employment of affinity chromatography using heparin-linked support material. Described is a simplified three step procedure for extracting basic FGF from either mammalian brain or mammalian pituitary tissue. Salt precipitation, e.g., with ammonium sulfate is used to provide a partially purified precipitate that is then subjected to ion-exchange chromatography, e.g., using a Carboxymethyl-Sephadex column. Substantially pure basic FGF fractions are then obtained by fractionating the further partially purified fractions using affinity chromatography on a heparin-linked support e.g., Heparin-Sepharose.
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申请公布号 |
US4785079(A) |
申请公布日期 |
1988.11.15 |
申请号 |
US19860940524 |
申请日期 |
1986.12.10 |
申请人 |
THE SALK INSTITUTE FOR BIOLOGICAL STUDIES |
发明人 |
GOSPODAROWICZ, DENIS;BAIRD, ANDREW;BOHLEN, PETER |
分类号 |
C07K14/50;(IPC1-7):C07K3/28 |
主分类号 |
C07K14/50 |
代理机构 |
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代理人 |
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地址 |
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