METHOD FOR MEASURING HYDROGEN PEROXIDE IN SAMPLE

摘要

PURPOSE:To eliminate the disturbance of measurement by hydrogen peroxide so that the hydrogen peroxide in a sample can be exactly determined by adding a reducing agent to the reagent to be used for a measuring system to decompose away the hydrogen peroxide existing in the reagent. CONSTITUTION:Sodium borohydride which is a reducing agent is added to luminol which is a luminescent reagent and after the reagent is rested until foaming ends, the reagent is filtered to obtain a soln. A. 0.5ml soln. A and 0.5ml microperoxidase are added to 0.1ml hydrogen peroxide soln. which is the measuring sample, and the quantity of the emitted light generated at this time is measured by a luminometer. The materials for disturbing the measurement existing in the reagent soln. to be used for the measuring system are thereby removed. The influence of background is thus not exhibited until the hydrogen peroxide decreases to the low concn., by which the exact measurement is carried out down to the low concn. region.