| 摘要 |
NEW MATERIAL:Polypeptides having 29K and 31K molecular weight contained in fibronectin fragment formed by treatment with trypsin, having insulin bond activity. USE:A remedy for diabetes, treatment promoter for wounds and a preventive for skin aging. PREPARATION:For example, fibronectin is digested with a small amount of trypsin at 35 deg.C for 60min, the reaction solution is subjected to affinity chromatography by an insulin sepharose column containing immobilized insulin and the adsorbed substance is treated with 1M eluting solution of NaCl and 4M eluting solution of urea to give 1M NaCl eluted fraction and 4M urea eluted fraction. Then these fractions are subjected to SDS-polyacrylamide electrophoresis so that it is found that polypeptide having 29K molecular weight exists in the 1M NaCl eluted fraction and polypeptide having 31K molecular weight exists in the 4M urea eluted fraction.
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