发明名称 |
FREMGANGSMAADE TIL ISOLERING OG RENSNING AF IMMUNOLOGISK AKTIVT POLYRIBOSYLRIBITOLPHOSPHAT |
摘要 |
The invention provides methods for the purification of polyribosyl ribitol phosphate (PRP) from Haemophilus influenzae type b cultures, as well as novel forms of immunologically active PRP obtainable by such methods and combined vaccines containing immunologically active PRP and Bordetella pertussis antigens. The purification methods involve removing contaminants to a low level by treating the PRP in the form of a hexadecyltrimethyl ammonium bromide complex with a calcium phosphate adsorbent (hydroxylapatite) in a phosphate buffer, and may be practiced either as a batch process or as a column process. The purified PRP thus obtainable is immunologically active and may be used with B. pertussis antigens to form a combined vaccine which elicits anti-PRP antibody and antipercussis antibody formations in young animals. This sera with anti-PRP antibody exhibits a strong bactericidal activity. |
申请公布号 |
DK154327(B) |
申请公布日期 |
1988.11.07 |
申请号 |
DK19780004795 |
申请日期 |
1978.10.27 |
申请人 |
AMERICAN CYANAMID COMPANY |
发明人 |
JOSEPH S.-C. KUO |
分类号 |
C12N1/20;A61K35/74;A61K39/095;A61K39/102;A61K39/116;A61K39/295;C12P1/04;(IPC1-7):A61K39/102;C12P19/04 |
主分类号 |
C12N1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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