| 摘要 |
Assay method for immunochemically detecting Chlamydia infections in mammals and birds, wherein bacteria of the genus Chlamydia potentially present in a sample are lysed in a lysing medium. The characteristic feature of this method consists in that an antigen released in the lysis step is adsorbed physically to a solid phase, and that a monoclonal anti-Chlamydia antibody preparation is contacted with the solid phase to which the antigen has been adsorbed. Conditions are arranged in such a manner that antibody-active components in the preparation are enabled to immunochemically bind to Chlamydia antigen that has been adsorbed to said solid phase. In a preferred embodiment the anti-Chlamydia antibody preparation is added to the lysing medium, thus avoiding a separation step. The characteristic feature of the anti-Chlamydia antibody active components of the antibody preparation is that they consist of a plurality of antibody molecules and/or label groups bound covalently together. |
