METHOD FOR PRODUCING CYTIDINE MONOPHOSPHATE OF 5- ACETAMIDO-3,5-DIDEOXY-D-GLYCERO-D- GALACTONONULOSAMINIC ACID

摘要

Cytidine monophosphate of 5-acetamido-3,5-dideoxy-D-glycero-D-galactononulosaminic acid is produced by an improved method by the condensation of cytidine triphosphate (CTP) with N-acetylneuraminic acid (NANA), catalyzed by the enzyme CMPtransferase (CMP-acylneuraminate synthase) (EC 2.7.7.43), either (A) in the presence of a thiocarboxylic acid or nitroimidazole stabilizer or (B) using the enzyme isolated from the E. coli strain CRC 1482. The product can be used as an active substance in pharmaceutical compositions for use in the therapy of pathological states related to disturbances of the nervous stimulus in the central (CNS) and peripheral (PNS) nervous system.