| 摘要 |
PURPOSE:To produce pyruvic acid in high efficiency, using fermentation process, by culturing a microbial strain belonging to Torulopsis genus, requiring arginine for growth and capable of producing pyruvic acid in a medium. CONSTITUTION:Torulopsis glabrate IFO 0005 is used as a mother strain and is treated with ethylmethane sulfonate to obtain an L-argininerequiring variant Torulopsis glabrate X-15 (FERM BP-1423). The obtained variant strain is cultured in a medium containing carbon source, nitrogen source, inorganic salts, vitamins, etc., at 22-32 deg.C while controlling the pH of the medium to 3-7 with an alkali. Pyruvic acid can be separated from the cultured product. |
