| 摘要 |
PURPOSE:To obtain the titled substance having high purity from an easily available raw material, by carrying out tissue culture of a cell originated from human tissue and separating a substance having specific molecular weight, isoelectric point, stability and amino acid composition and capable of prolonging blood coagulation time from the cultured cell. CONSTITUTION:Cell originated human tissue (e.g. pulmonary cell of human fetus) is subjected to tissue culture in a medium containing bovine fetus serum, etc., and the objective anticoagulant substance having the following properties is separated from the cultured cell by extraction, etc. Molecular weight (SDS- PAGE method in reduced state), 34,000+ or -2,000; isoelectric point (isoelectric column electrophoresis using ampholite), 4.7+ or -0.1; stability, inactivated by heat- treatment at 50 deg.C for 30min, stable at pH 4-10, stable in plasma at 37 deg.C for 30min; activity, prolongs recalcification coagulation time, prothrombin time an activated part thromboplastin time; amino acid analysis, positive to Asp, Thr, Ser, Glu, Pro, Gly, Ala, Cys, Val, Met, Ile, Leu, Tyr, Phe, His, Lys and Arg and negative to sugar. |
