Process for concentrating and separating trypsin inhibitor and kallidinogenase in human urine

摘要

Two components, trypsin and kallidinogenase, in human urine are concentrated simultaneously by allowing human urine at neutral pH, collecting bubbles thus formed to obtain the concentrate of the two components, adjusting the concentrate to weak acidity, contacting the acidified concentrate with chitosan to allow the two components to be adsorbed onto chitosan, eluting the components from the adsorbent with aqueous ammonia solution, and neutralizing and heating the eluate at about 60 DEG C. for about 10 hours to make the eluate virus-free, followed by separating the components from the eluate.