| 摘要 |
A method of solid state immunoassay incorporating a luminescent label for the quantitation of antigen, hapten or antibody analyte in a measured volume of a liquid sample comprises the following steps:
reacting the analyte or an analyte containing reaction product with or in competition with or for an immunoreactant which is attached to (i) a plurality of water insoluble particles of about 10 mu m or less in size, or (ii) cells, the particles or cells being in a substantially suspended state;
<??>concentrating by microfiltration the water insoluble particles, or cells, which have, or which through subsequent reaction will have, a luminescent label attached thereto, to a volume substantially less than the volume of the liquid sample; and
measuring the luminescence of substantially all of the luminescent label attached to the concentrated water insoluble particles or cells. |
