Method of quantitatively measuring an oxidative substance using triphenyl methane type leuco-pigment as a coloring substance

摘要

Disclosed herein is a method of quantitatively measuring an oxidative substance with avoiding influences of a coloring-interfering substance in the quantitative measurement of the oxidative substance by using a triphenyl methane type leuco coloring matter as a coloring reagent. Disclosed herein is also such an oxidative quantitative measurement in which the coloring sensitivity can be further adjusted. In order to avoid the influences of the coloring-interfering substance, there is employed at least one kind of (i) uricase, (ii) an anionic surface active agent and (iii) a metal chelate compound. As the triphenyl methane type leuco coloring matter, use may be made of a compound of the general formula (I): (I) wherein R1, R2, R3 and R4, which may be the same as or different from one another, represent a hydrogen atom or a lower alkyl group, and X1 and X2, which may be the same as or different from each other, represent a hydrogen atom, -SO3M1, -COOM2, -O(CH2)mSO3M3, -O(CH2)nCOOM4 or -N(R5) (R6) in which M1, M2, M3 and M4 represent a hydrogen atom, an alkali metal ion or NH4+, R5 and R6 independently represent a hydrogen atom or a lower alkyl group, and m and n represent an integer of 2-4. Azide compound is further added or the compound represented by the general formula (I) is included with cyclodextrin or modified dextrine to adjust coloring sensitivity.