| 摘要 |
PURPOSE:To obtain high-purity viruses free from impurities in high efficiency, by culturing HmLu-1 cell in serumfree medium and culturing Akabane virus using the cultured cell as a host. CONSTITUTION:HmLu-1 cell is cultured in a DME/F12 medium added with 10% NBCS until the proliferation reaches saturation. The NBCS is slowly removed from said cell culture system and the system is added with a cell proliferation factor ITES to convert the cell to serumfree state. The serumfree HmLu-1 cell is scattered on a Petri dish, cultured for 2 days in serumfree state, adsorbed with a virus such as subspecies of Akabane virus NBE-9, SAB74, JaGar-39, Ibaraki virus, Nakayama virus or bovine ephemeral fever virus. After washing the surface of the cell, said virus is cultured in DME/F12 medium.
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