| 摘要 |
PURPOSE:To enable accurate immunological measurement of hemoglobin in excrement, by making a glycosidase type bacteriolysis enzyme act upon a sample. CONSTITUTION:An excrement sampling spoon is coated with a buffer containing an antihuman hemoglobin antibody by being immersed thereinto for 24hr. The spoon sticks into excrements to sample a fixed amount thereof. On the other hand, NaN3 and a bacteriolysis enzyme such as lysozyme are added to an agar solution to gelatinize. The excrement sampling spoon having excrements sampled is inserted into the agar gel and allowed to stand for a fixed time. Then the spoon is taken out and washed to be immersed into an alkali phosphodase labelled antibody solution. After washed, the spoon is immersed into a phenyl phosphate substrate liquid to incubate and after the addition of a coloring liquid, reaction is stopped to measure the degree of coloration.
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