| 摘要 |
A method for detecting the MB isoenzyme of creatine kinase in a biological fluid characterised in that it comprises: (a) forming a mixture of (i) a sample of the biological fluid, (ii) an antibody to the B monomer of creatine kinase, the said antibody being bound to a solid support, and (iii) a labelled, monoclonal antibody to the MB isoenzyme of creatine kinase; (b) incubating the mixture; (c) separating the solid support from the mixture; and (d) detecting the amount of label associated with the solid support is disclosed. A monoclonal antibody characterised in that it is produced by a murine-derived hybrid cell line, the said antibody being capable of specifically binding to at least one antigenic determinant of the MB isoenzyme of human creatine kinase and being of the IgG1 type is also disclosed. A murine-derived hybrid cell line ATCC HB 9389 or HB 9388 and mutants thereof are further disclosed. More particularly, a single incubation CKMB assay is provided which employs an anti-CKB capture antibody bound to magnetic particles and an anti-CKMB tracer antibody labelled with an acridinium ester. The assay achieves a minimal detectable dose of at least 1 mg/l CKMB for incubations as short as 10 minutes at room temperature, and is not subject to interference due to the presence of macro-CK-1 or high levels of CKMM and CKBB in serum samples. In accordance with certain preferred embodiments of the invention, the anti-CKMB tracer antibody is a mouse monoclonal antibody of the IgG1 type. The present assay is faster, simpler to use and less subject to interference than prior art assays and yet maintains the sensitivity thereof. |
