BIOTINYL REAGENT AND BIOTINYLATION USING SAID REAGENT

摘要

NEW MATERIAL:A compound shown by formula I (X is -COO<->, -CONH2 or H; n is 1-10). EXAMPLE:A salt of N-d-biotinyl-3-nitro-2-pyridylsulfenyl-L-cysteine N. USE:A biotinyl reagent useful for avidin-biotin-N-system and important in the filed of purification of preformed chemical mediator as well as immune analysis, in vivo microanalysis and chemical analysis. PREPARATION:A compound shown by formula II is reacted with d-biotinyl-N hydroxysuccinimide ester. The starting raw material shown by the formula II is obtained by treating N-t-butoxycarbonyl-S-3-nitro-2-pyridylphenylcysteine shown by formula III with an acid.