Device for quantitative endpoint determination in immunofluorescence using microfluorophotometry

摘要

The present invention discloses a device and a process for quantitation of Toxoplasma gondii and Treponema pallidum antibody titer in a biological sample by immunofluorescent photometric microscopy. The process comprises: (a) reacting a specimen of said sample with an immunofluorescent reagent in a mounting medium containing a protective agent in an amount sufficient to reduce fading of a fluorescent reaction product less than 25% of initial fluorescent intensity; (b) localizing the specimen under transmitted, visible light; (c) reducing the effect of counterstain intensity by filters in emission light path; (d) measuring the sample using a fast shutter; (e) calibrating the photometer used in said microscopy by a stable fluorophore; (f) recording intensity of fluorescence of said specimen compared to standard negative and positive controls; (g) reducing effect of polar staining by substracting the corrected intensity of corresponding dilution of the negative control from the sample reading; and (h) assigning a numerical endpoint for serum antibody levels against Toxoplasma gondii or Treponema pallidum.