发明名称 |
Proteinaceous substance showing antitumorous action and its preparing method |
摘要 |
A proteinaceous substance having the following biochemical characteristics: (A) a strong cytolytic activity against mouse tumor cells L-929, but not cytotoxic activity for normal cells such as human fetus fibroblasts, human adult skin fibroblasts and chinese hamster fibroblasts (Don, V-79) and therefore a high tumor specificity: and (B) a strong anti-tumor and immunogenic activity against Meth-A sarcoma. When said substance is injected intravenously to the syngenic BALB/C mice inoculated with Meth-A sarcoma at a quantity of more than 72 units head, not only hemorrhagic necrosis, but also complete degradation is observed. Thereafter, even if it is attempted again to transplant the Meth-A sarcoma cells under the skin of Mice cured completely, successful plantation is not observed because of an acquired immunity: (C) the substance has no pyrogenetic action after intravenous injection to rabbits: (D) a molecular weight of 45,000+/-5,000 (determined by the gel filtration method with Sephacryl S-200): and (E) an isoelectric point pI of 4.8+0.3: and a method for its manufacture.
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申请公布号 |
US4777241(A) |
申请公布日期 |
1988.10.11 |
申请号 |
US19860885737 |
申请日期 |
1986.07.21 |
申请人 |
KYORIN PHARMACEUTICAL CO., LTD. |
发明人 |
IRIKURA, TSUTOMU;TAKAGI, KOICHI;HOSOMI, JIRO;MURAYAMA, SATOSHI;SAITO, KOJI;OKAZAKI, TAKASHI |
分类号 |
G01N33/574;A61K35/12;A61K38/00;A61K39/395;A61P35/00;C07K1/22;C07K14/005;C07K14/195;C07K14/52;C07K14/525;C07K16/24;(IPC1-7):C07K15/00 |
主分类号 |
G01N33/574 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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