DNA CODING BOVINE PHOSPHOLIPASE A2

摘要

PURPOSE:To obtain a DNA coding bovine pLA2, by preparing a cDNA bank from bovine pancreas and separating from the bank using a synthetic DNA oligomer as a probe. CONSTITUTION:A cDNA library prepared from the mRNA of bovine pancreas is integrated into a plasmid, an E.coli is transformed with the plasmid and the transformant is proliferated. The proliferated microorganism is subjected to colony hybridization using a synthetic oligomer as a probe to obtain two hybridizing clones. A DNA coding bovine pLA2 (phospholipase A2) having the base sequence of formula is separated from the plasmid DNA of the clone. A plasmid DNA, pBLA2-18.3 containing said DNA is introduced into E.coli MC 1061 to obtain a transformant E.coli MC 1061 [pBPLA2-18.3 (FERM p-9298)]. The objective DNA is separated by proliferating the obtained E.coli.