| 摘要 |
Prepn. of L-glutamic acid by culture of a mutant, Brevibacterium sp. strain CS 850815 (KFCC 10183) having a resistance against azaserine and sulfamylamide comprises (a) inoculating 1 liter of preculture of a mutant KFCC 10183 into a 3 liter far-fermentor contg. 13 liter medium consisting of 100 sugar beet molasses, 1 MgSO4.7H2O, 0.4 FeSO4.7H2O, 0.01 MnSO4.7H2O, 0.01 thiamine.HCl, 30 CSL, 0.02 alanine, 0.02g glycine and 300 μg biotin/dl and culturing at pH 7.8, 1 vvm, and 350 rpm; and (b) adding Tween 60 up to 0.5g/1 after 6hr of incubation and culturing for further 30hr to give 65% yield based on sugar.
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