| 摘要 |
PURPOSE:To enable efficient production of the titled compound, by transducing part of Ri plasmid of Agrobacterium rhizogenes to a cell of plant of the family Boraginaceae, transforming the cell to give a hair root, cultivating the hair root in a medium and continuously recovering the titled secreted compound from the medium. CONSTITUTION:A stem, root, leaf, etc., of a plant such as Lithosperumum erythrorhizon belonging to the family Boraginaceae is treated with Agrobacterium rhizogenes, part (T-DNA) of Ri plasmid of the bacterium is transduced to nucleus DNA of the plant cell to give a transformed hair root. About 10mg-1g (fresh weight) of the hair root is transplanted in a liquid medium containing about 1-120g/l, about 0.1muM-100mg inorganic component, further about 0.01-10muM plant hormone, about 0.1-100mg/l vitamins and about 0.1-100mg/l amino acids, cultivated at a dark place at about 10-35 deg.C, preferably about 23-28 deg.C and a naphthoquinone compound secreted in the medium from the hair root is continuously recovered. |
