DIFFERENTIEL BINDING AF POTENTIELT SENSITIVE MATERIALER TIL LYMPHOCYTTER

摘要

The method comprises contacting a suspension of viable lymphocytes with a solution containing a membrane potential sensitive fluorescent dye. The contact between the lymphocyte suspension and the MPS dye is maintained for sufficient time to permit labelling of the lymphocytes by the MPS dye material. The labelled lymphocytes are passed individually through a focused source of excitation energy causing any MPS dye material to fluoresce. The fluorescent emission from each cell is recorded as a pulse, and the number of pulses of each intensity is recorded. The distribution of the number of pulses versus the fluorescence intensity is bimodal. The first peak includes pulses of low fluorescence intensity, and the second peak comprises pulses of high fluorescence intensity. The ratio of the total counts of low fluorescence intensity to the total counts of high fluorescence intensity produces a factor which distinguishes lymphocytes derived from a body afflicted with malignancy from bodies which are not afflicted with malignancy.